Beauveria bassiana haplotype determination based on nuclear rDNA internal transcribed spacer PCR-RFLP
نویسندگان
چکیده
منابع مشابه
Molecular Identification and Differentiation of Fasciola Isolates Using PCR- RFLP Method Based on Internal Transcribed Spacer (ITS1, 5.8S rDNA, ITS2)
BACKGROUND In this study, we used both ITS1 and ITS2 for molecular identification of Fasciola species. METHODS The region between 18S and 28S of ribosomal DNA was used in PCR-RFLP method for molecular identification of Fasciola species. Ninety trematodes of Fasciola were collected during abattoir inspection from livers of naturally infected sheep and cattle from Khorasan, East Azerbaijan, and...
متن کاملA case of Beauveria bassiana keratitis confirmed by internal transcribed spacer and LSU rDNA D1–D2 sequencing
We describe a case of fungal keratitis due to Beauveria bassiana in a farmer with Fuchs' dystrophy, treated with amphotericin B. Surgery with penetrating keratoplasty was necessary to resolve the lesions. Susceptibility testing and molecular sequencing permitted the identification and treatment of this rare aetiological agent of invasive fungal disease.
متن کاملmolecular identification and differentiation of fasciola isolates using pcr- rflp method based on internal transcribed spacer (its1, 5.8s rdna, its2)
background : in this study, we used both its1 and its2 for molecular identification of fasciola species. methods : the region between 18s and 28s of ribosomal dna was used in pcr-rflp method for molecular identification of fasciola species. ninety trematodes of fasciola were collected during abattoir inspection from livers of naturally infected sheep and cattle from khorasan, east azerbaijan, a...
متن کاملMolecular phylogeny of Oncaeidae (Copepoda) using nuclear ribosomal internal transcribed spacer (ITS rDNA)
Copepods belonging to the Oncaeidae family are commonly and abundantly found in marine zooplankton. In the Mediterranean Sea, forty-seven oncaeid species occur, of which eleven in the Gulf of Naples. In this Gulf, several Oncaea species were morphologically analysed and described at the end of the XIX century by W. Giesbrecht. In the same area, oncaeids are being investigated over seasonal and ...
متن کاملComplete nucleotide sequence of Beauveria bassiana 5.8s rRNA coding gene and flanking internal transcribed spacers.
The nucleotide sequence of two clones of Beauveria bassiana in 5.8s rRNA coding gene and ITS regions were completely sequenced. The overall sequence similarity of these two clones is 96%. The identities of internal transcribed spacer (ITS) regions are 91 % (ITSI) and 100% (ITSII), respectively. Both of 5.8s rRNA sequences have 98% homology.
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ژورنال
عنوان ژورنال: Mycological Research
سال: 2002
ISSN: 0953-7562
DOI: 10.1017/s0953756201005305